Summary
The use of molecular assays to assess the presence of species using environmental DNA (eDNA) and RNA (eRNA) as analytes has diversified as the field of environmental surveillance advances. These methods can be used, for example, to screen for pest species as part of biosecurity measures and risk management, to screen for threatened species as part of development requirements, or for biodiversity monitoring purposes. To ensure that testing results are reliable, it is imperative to develop and validate molecular assays using stringent quality standards that can minimise the potential for false negative and false positive results.
Authors:De Brauwer M. Chariton A.A. Clark L.J. Cooper M.K. DiBattista J. Furlan E. Giblot-Ducray D. Gleeson D. Harford A. Herbert S. MacDonald A.J. Miller A. Montgomery K. Mooney T. Noble L.M. Rourke M. Sherman C.D.H. Stat M. Suter L. WeK.M.st White N. Villacorta-Rath C. Zaiko A. Trujillo-Gonzalez A.
Publisher: National eDNA Reference Centre, Canberra
Published in: 2022
Category 6: Report
Program area: East Antarctica Monitoring Program
De Brauwer M., Chariton A.A., Clark L.J., Cooper M.K., DiBattista J., Furlan E., Giblot-Ducray D., Gleeson D., Harford A., Herbert S., MacDonald A.J., Miller A., Montgomery K., Mooney T., Noble L.M., Rourke M., Sherman C.D.H., Stat M., Suter L., WeK.M.st , White N., Villacorta-Rath C., Zaiko A., Trujillo-Gonzalez A.
(2022)
Environmental DNA test validation guidelines,
National eDNA Reference Centre, Canberra
33pp;
https://repository.oceanbestpractices.org/handle/11329/2143 ALSO https://sednasociety.com/publications/;
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SUTER, Dr Leonie
Other Antarctic Location (Territory of Heard Island and McDonald Islands), Australia (or home country), Marine Science