Summary
The major phospholipids of Halorubrum lacusprofundi grown at 25 1C were archaeol phosphatidylglycerol, archaeol phosphatidylglycerylsulphate and archaeol phosphatidylglycerylphosphate methyl ester.Glycolipids included a monoglycosyl archaeol and the sulphate ester of a diglycosyl archaeol.Cul tures grown at 12 1C contained the same suite of phospho- and glycolipids, with the addition of a series of unsaturated analogues with up to six double bonds. The patterns of unsaturation were similar for all the phospholipid series, but a different pattern occurred in the glycolipids.The analytical techniques used in this study allow facile detection of unsaturated archaeal cell membrane lipids that are degraded by commonly used chemical derivatization procedures.